Regulatory

Part:BBa_K3032034:Design

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-14)


ColE based PpsR2 promoter (2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PpsR2 DNA binding sequence was carefully combined with ColE promoter sequence in order not to affect promoter's activity.


Source

This promoter sequence based on DOI:10.1021/acssynbio.7b00289.


References